ADCY5 ELISA Kit

Catalog No. ABIN2042779

Human ADCY5 ELISA Kit Colorimetric assay for quantification of Human ADCY5.

Quick Overview for ADCY5 ELISA Kit (ABIN2042779)

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ADCY5 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.124 ng/mL

Application Details

Plate: Pre-coated

Protocol: Adenylate Cyclase 5 (ADCY5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 5 (ADCY5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 5 (ADCY5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 5 (ADCY5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 5 (ADCY5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ADCY5

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma